FIGURE

Fig. S1

ID

ZDB-FIG-100723-21

Publication

Iida et al., 2010 - Metalloprotease-dependent onset of blood circulation in zebrafish

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Fig. S1

Confocal Images of Erythroid Cells Invading into the Vasculature

This figure relates to Figure 1 and 2.

(A and B) Time-lapse imagings of their invasion into the PCV in a wild-type embryo. In (A), endothelial cells transiently encapsulate an erythroid cell invading into the PCV. Scale bar represents 25 μm. In (B), an erythroid cell adheres to the PCV with protrusions after invasion. Scale bar represents 5 μm.

(C) An electron micrograph of showing adherence between the endothelial cells and between the erythroid and endothelial cells (C′) and a tiny membrane protrusion (black arrow in C″). Scale bar represents 10 μm.

(D-G) Confocal images of live embryos injected with ortho-phenanthroline (D and E) or GM6001 (F and G) and showing normal invasion of gata1:mRFP⁺ cells (color dots) into the vasculature, but a dramatic accumulation inside the blood vessels in the trunk in those embryos. Scale bar represents 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.