FIGURE

Fig. 2

ID

ZDB-FIG-100719-21

Publication

Say et al., 2010 - A Functional Requirement for PAK1 Binding to the KH(2) Domain of the Fragile X Protein-Related FXR1

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Fig. 2

The KH2 Domain of FXR1 Is Implicated in PAK1 Binding

(A) Constructs encoding the N-terminal domain of human FMR1(1–380), FXR1(1–390), FXR2(1–380), or zebrafish Zf-FXR1(1–380) were expressed in COS-7 cells and lysates passed through GSH Sepharose preloaded with AID sequences derived from human PAK1, PAK2, or PAK3 as indicated. The presence of fragile X proteins was shown by western blotting with anti-HA. The blot shown represents a single panel.

(B) A schematic of the domain structure of PAK kinases showing the disposition of Cdc42-binding CRIB domain, autoinhibitory AID, and PIX-binding region. The region of PAK1 required for binding FXR1 is shown, with those residues that are identical between the three isoforms shaded.

(C) The KH(2) domain of FXR1 is implicated in PAK1 binding. HA-tagged versions of fragile X-related proteins were co-expressed with the GST-PAK1-AID as shown. The presence of the HA-tagged protein (bottom) and in the GSH Sepharose pull-down (top) indicates that KH2 FXR1(284–380) is sufficient to allow PAK1 binding. See the lane FXR2(1–296)-FXR1(284–380) designated chimaera 2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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