Whole-mount in situ hybridisation for dystroglycan. (A) 128-cell stage and (B) 1000-cell stage, indicating maternal expression. (C) Shield stage and (D,E) tailbud stage; posterior (D) and anterior (E) views, indicating expression in the chordamesoderm and developing brain. (F,G) Five-somite stage, posterior (F) and anterior (G) views. (H) Flat mount of a 12-somite embryo, showing widespread expression. (I) Sections through tailbud and hindbrain level of the 12-somite embryo in H. Expression in immature (upper section) but not mature (lower section) notochord is indicated (arrow). (J) Trunk of 24 hpf embryo. High expression in the hypochord is indicated (arrow).
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