Fig. 3

Meis1 protein levels are severely diminished in the posterior mesoderm of Pbx-depleted and meis1-morphant embryos. Shown are representative embryos following immunohistochemical staining with the P2A6 antibody (green; A, D, G, J) to visualize Meis1 protein levels, and Hoechst 33258 (red; B, E, H, K) to visualize nuclei in 14 hpf wild type (WT; A–C), meis1-morphant (D–F), Pbx-depleted (G–I), and Pbx-depleted; meis1-morphant (J–L) embryos. Flat-mount; dorsal view of posterior mesoderm; anterior to left. All embryos visualized under a 100x objective. In wild type (WT; A–C) embryos, α-Meis1 antibody staining is punctate, and colocalizes with Hoechst nuclear staining. meis1-morphant (D) and Pbx-depleted (G) embryos exhibit severely decreased α-Meis1 antibody staining when compared to WT embryos (A). α-Meis1 antibody staining is further abolished in Pbx-depleted; meis1-morphant embryos (J). α-Meis1 antibody staining does not colocalize with Hoechst nuclear staining in Pbx-depleted embryos (G–I).