FIGURE

Fig. 6

ID

ZDB-FIG-100319-33

Publication

Degenhardt et al., 2010 - Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expression

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Fig. 6

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Fig. 6

Analysis of transgenic zebrafish shows that ECR2 directs expression to the dorsal neural tube and that this expression requires Lef1/TCF sequences. (A1–A4) Lateral view of a 1-dpf zebrafish embryo that is stably transgenic for Sox10:EGPF and transiently transgenic for ECR2:mCherry demonstrates strong dorsal neural tube expression (yellow arrowheads). (B1–B4) Dorsal view of a similarly injected embryo as shown in column A. (C1–C4) Lateral view of a 24-hpf zebrafish embryo that is stably transgenic for Sox10:EGPF and injected with an ECR2^ΔLef:mCherry transgene shows that mutations in the Lef1/TCF sequences abrogates expression. (D1–D4) Lateral view of a 24-hpf zebrafish embryo that has been injected with both ECR2:EGFP and ECR2^ΔLef:mCherry transgenes shows EGFP expression in the dorsal neural tube (yellow arrowheads). Despite effective transposase activity, no mCherry expression is detected. Scale bar = 500 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 339(2), Degenhardt, K.R., Milewski, R.C., Padmanabhan, A., Miller, M., Singh, M.K., Lang, D., Engleka, K.A., Wu, M., Li, J., Zhou, D., Antonucci, N., Li, L., and Epstein, J.A., Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expression, 519-527, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.