Fig. 9
Inhibition of cell proliferation has little effect on spinal cord elongation. A-C′: Sagittal sections through the tailbud of control (A-C) and drug-treated (A′-C′) 4 somites (som; A,A′), 14 som (B,B′), and 20 som (C,C′) embryos, labeled with α-Sox3C (neural tissue, pink), α-PH3 (mitotic, nuclear marker, green), α-β-catenin (cell surface marker, green), and DAPI (4′,6-diamidine-2-phenylidole-dihydrochloride; nuclear marker, blue). KV, Kupffer′s vesicle; som, somites. Scale bar = 20 μm. |