FIGURE

Fig. 3

ID

ZDB-FIG-100309-45

Publication

Peterson et al., 2010 - Hedgehog signaling and laminin play unique and synergistic roles in muscle development

Other Figures

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Fig. 3

Hh signaling is required indirectly for the recovery of fast-twitch fibers in laminin mutant embryos. B-I: Side views, anterior left, dorsal top, 48-hpf embryos treated with EtOH or CyA at the 10-somite stage and stained with F59 (green) to visualize slow muscle fibers and phalloidin (red) to visualize actin. A: Cartoon describing expected results if Hh signaling is directly or indirectly required. B-E: Anterior somites are shown. Panels numbered 1 are 3-D projections. Panels numbered 3 are higher-magnification views of individual focal planes. Note the presence of slow-twitch fibers and elongated fast fibers in all conditions. F-I: Posterior somites are shown. The boxes in panels numbered 1 and 2 correspond with the higher-magnification medial focal plane shown in panels numbered 3. Slow-twitch fibers are present in EtOH-treated embryos (F, G) but are reduced and/or absent in posterior somites in CyA-treated embryos (H, I). Note that defects in fast fiber elongation correlate with the lack of slow fibers in the posterior of CyA-treated lam gamma1 mutant embryos (I).

Expression Data

Antibody:	Ab-F59
Fish:	WT lamc1^{ti263a/ti263a}
Condition:	chemical treatment: Cyclopamine
Anatomical Term:	slow muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT lamc1^{ti263a/ti263a}
Condition:	chemical treatment: Cyclopamine
Observed In:	slow muscle cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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