FIGURE

Fig. 1

ID

ZDB-FIG-100114-10

Publication

Dovey et al., 2009 - Oncogenic NRAS Cooperates with p53 Loss to Generate Melanoma in Zebrafish

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Fig. 1

NRAS^Q61K causes hyperpigmentation in zebrafish. (A) Schematic representation of the transgene. (B) Animals injected with the NRAS construct develop nevi. (C) Hyperpigmentation in the NRAS1 and NRAS2 transgenic animals disrupts the normal pigment pattern. (D) Transgene copy number of NRAS1 and NRAS2 animals. Primers directed against the mitfa promoter were used. Wild-type fish were also used in this comparison and normalized to two copies per genome. (E) Relative transgene expression as measured by EGFP mRNA in both transgenic lines. (F) Low-grade melanocytic lesion observed in NRAS1 transgenic animals. Scale bar is 10μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf170Tg zf171Tg
Observed In:	pigmentation
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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