FIGURE

Fig. S1

ID

ZDB-FIG-091215-54

Publication

Alvarez et al., 2009 - Selective Inhibition of Retinal Angiogenesis by Targeting PI3 Kinase

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Fig. S1

Intraocular Injection of LY294002 in zebrafish larvae. A) ∼5 dechorionated zebrafish larvae (48 hpf in the figure) are immobilized in CyGEL at RT and oriented with left eye facing upwards. Dechorionation is extremely important as non-dechorionated larvae collapse in contact with CyGEL. B) Intraocular injection (0.1?0.2 μl/eye) is performed with a heat pulled glass capillary. C) After intraocular injection the Petri dish is placed on a chilled ice pad to melt CyGEL and larvae are transferred gently but immediately to a plate with RT embryo medium for recovery.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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