FIGURE

Fig. 3

ID

ZDB-FIG-091016-33

Publication

Green et al., 2009 - A gain of function mutation causing skeletal overgrowth in the rapunzel mutant

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Fig. 3

Morpholino knockdown of rpz suppresses the homozygous rapunzel phenotype. Heterozygous rapunzel adult zebrafish were intercrossed and the resulting embryos injected at the 1–2 cell stage with either a morpholino targeting the start site of rpz (MOrpz) (A), a morpholino targeting rpz2 (MOrpz2) (B) or a rpz 5 base pair mismatched control morpholino (MOrpz_5bm) (C). A. Injection of MOrpz suppresses the homozygous embryonic rapunzel phenotype. The rapunzel homozygous phenotype was not suppressed in embryos injected with MOrpz2 (B) or MOrpz_5bm (C). PCR, DdeI digestion and electrophoresis confirmed that the embryos in A–C are all homozygous for the rapunzel^c14 allele (not shown).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rpz^c14/c14 + MO1-rpz rpz^c14/c14 + MO1-rpz2
Knockdown Reagents:	MO1-rpz MO1-rpz2
Observed In:	pericardium whole organism whole organism anterior-posterior axis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Green, J., Taylor, J.J., Hindes, A., Johnson, S.L., and Goldsmith, M.I., A gain of function mutation causing skeletal overgrowth in the rapunzel mutant, 224-234, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.