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Fig. 7

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ZDB-FIG-090714-16

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Coffin et al., 2009 - Extracellular divalent cations modulate aminoglycoside-induced hair cell death in the zebrafish lateral line

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Fig. 7

Amiloride blocks drug uptake and the resulting loss of hair cells. (A) Amiloride attenuates short-term (10 min) GTTR uptake (right) as compared to GTTR uptake without amiloride (left). Hair cell nuclei are labeled with YO-PRO-1 (blue), scale bar = 5 μm and applies to both panels. (B) 1 mM amiloride prevents hair cell loss from acute neomycin or gentamicin exposure (unpaired t-tests, p > 0.05 for amiloride + aminoglycoside as compared to mock-treated controls). Black bars = no amiloride, gray bars include 1 mM amiloride. Controls show that amiloride alone does not alter DASPEI scores (unpaired t-test, p > 0.05). Error bars in (B) are ±1 SD. All experiments shown here were conducted in standard EM (∼1 mM Ca²⁺).

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Acknowledgments

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Reprinted from Hearing Research, 253(1-2), Coffin, A.B., Owens, K.N., Raible, D.W., and Rubel, E.W., Extracellular divalent cations modulate aminoglycoside-induced hair cell death in the zebrafish lateral line, 42-51, Copyright (2009) with permission from Elsevier. Full text @ Hear. Res.