Fig. 3

moz is required for most hox1-4 expression domains. Lateral (A-D,G-H,M-V) and dorsal (E-F,I-L) views of Hox expression in wild type (A,C,E,G,I,K,M,O,Q,S,U) and moz mutants (B,D,F,H,J,L,N,P,R,T,V) at 33 hpf (A-F), 11 hpf (I,J) and 48 hpf (G,H,K-V). (A,B) hoxa2b expression, which is present in arches two and three of wild type (A), is undetectable in these arches in moz mutants (B). (C,D) hoxb2a expression normally present in the second arch (C), is barely detectable in the moz mutant second arch (D). (E,F) hoxa2b expression in the hindbrain is reduced in moz mutants (G,H) hoxa2b expression in second arch CNC is severely reduced (I,J) Initiation of hoxa2b expression at 11 hpf occurs normally in moz mutants. (K,L) hoxa1a in the ventral forebrain and midbrain, and anterior hindbrain, is unaffected in moz mutants (M-V) Graded affect on hoxb1a-b5a gene expression in moz mutants. (M,N) hoxb1a expression in r4 is largely abolished in moz mutants. (O,P) hoxb2a expression in r3-5 is downregulated in moz mutants, and second arch expression (arrow) is now undetectable. (Q,R) hoxb3a expression in the caudal hindbrain is reduced, and pharyngeal arch expression in arches 3-5 (arrow) is undetectable. (S,T) hoxb4a expression is mildly reduced in the posterior hindbrain and pharyngeal arches 4-6 (arrow). (U,V) hoxb5a expression appears unaffected in moz mutants. Scale bars: 50 μm.