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Fig. 3

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ZDB-FIG-090520-10

Publication

Thisse et al., 1994 - Goosecoid expression in neurectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas

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Fig. 3

Localization of gsc RNA in cyclops (cyc^b16) mutant embryos. (a) Side view of a whole mount cyclops embryo at 70% epiboly. Expression of gsc in the mesendoderm is reduced compared to wild-type control (Fig. 2a) (animal pole up; dorsal, right). (b) Dorsal view of the same embryo. gsc is confined to the most anterior part of the mesendodermal layer and encompasses only a portion of the wild-type gsc territory shown in (c) (anterior, top). (c) Dorsal view of a wild-type sibling embryo at 70% epiboly, showing gsc expression in the entire axial mesendoderm (compare to (b); see also Fig. 2a) (anterior, top). (d) Side view of a cyclops embryo at 95% epiboly, gsc expression is not detected in the ectoderm and is reduced in the mesodermal territory compared to wild-type expression shown in (e) (anterior, upper left). (e) Wild-type sibling embryo at 95% epiboly. Scale bars, 100 μm. Phenotypically mutant embryos were approximately one-quarter (23.4% = 55/235) of the total, as expected for simple Mendelian segregation.

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Acknowledgments

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Reprinted from Developmental Biology, 164, Thisse, C., Thisse, B., Halpern, M.E., and Postlethwait, J.H., Goosecoid expression in neurectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas, 420-429, Copyright (1994) with permission from Elsevier. Full text @ Dev. Biol.