FIGURE

Fig. 2

ID

ZDB-FIG-090408-79

Publication

Ackerman et al., 2009 - Cloning and spatiotemporal expression of zebrafish neuronal nicotinic acetylcholine receptor alpha 6 and alpha 4 subunit RNAs

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Fig. 2

Expression of chrna6 and chrna4 RNA in zebrafish. Reverse transcription polymerase chain reaction (RT-PCR) was used to determine when chrna6 and chrna4 nAChR subunit RNAs were expressed in embryos and larvae. Three micrograms of RNA from each age zebrafish (h, hpf; d, dpf) were reverse-transcribed and amplified using subunit-specific primers. The &beta-actin was also amplified for each age. As controls, PCR was performed using zebrafish RNA without reverse transcription (-r) or without cDNA (-d). The size of each PCR product was consistent with the size predicted by the cDNA sequences. The Φx 174 HaeIII DNA (Φχ) was used as a size marker.

Expression Data

Genes:	chrna4b chrna6
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	1k-cell to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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