Fig. 3
- ID
- ZDB-FIG-090128-2
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- Bauer et al., 1998 - Follistatin and Noggin are excluded from the zebrafish organizer
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Temporal expression pattern of zebrafish follistatin and noggin. (A) Developmental RT-PCR analysis for follistatin, noggin, and the ef1α (control). Neither noggin nor follistatin transcripts are detected before 80% epiboly. noggin signals are weak during late gastrulation and segmentation stages, but much stronger during primordia stages, with a maximum at day 2. follistatin signals are of comparable strength throughout all investigated stages from 80% epiboly onward, with a slight maximum at the 8- and 15-somite stages. (B) Developmental Northern blot hybridized with follistatin probe. Ten micrograms of stage-specific total RNA was loaded per lane and stained with ethidium bromide as a loading control. Consistent with the results of RT-PCR, expression is first detectable at 80% epiboly, reaches a maximum at day 1 of development, and then declines. Note the two transcripts of follistatin. The larger transcript is 3.5 kb, the shorter transcript approximately 2.4 kb. |
Reprinted from Developmental Biology, 204, Bauer, H., Meier, A., Hild, M., Stachel, S., Economides, A., Hazelett, D., Harland, R.M., and Hammerschmidt, M., Follistatin and Noggin are excluded from the zebrafish organizer, 488-507, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.