FIGURE

Fig. S5

ID

ZDB-FIG-090106-17

Publication

Jiang et al., 2008 - Exdpf is a key regulator of exocrine pancreas development controlled by retinoic acid and ptf1a in zebrafish

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Fig. S5

Exdpf is Specifically Required for Exocrine Pancreas Formation but not Gut or Liver

(A–C) Expression of GFP MP760GFP transgenic fish. (A) A control embryo injected with standard control morpholino. Note strong GFP expression in the pancreatic area at 24 hpf (arrow) and 2 dpf (arrowhead). (B) An example of embryo injected with exdpf mRNA (100 pg). Note strong GFP expression in the pancreatic area at 24 hpf (arrow) and 2 dpf (arrowhead). GFP expression in the developing gut is comparable to that in control embryo. (C) An example of embryo injected with exdpf morpholino (2ng of MO1^exdpf). Note no strong GFP expression in the presumed pancreatic area at 24 hpf (arrow). A weak GFP positive pancreatic bud can be observed at 2 dpf (arrowhead). GFP expression in the gut is still comparable to that in the control and exdpf RNA injected embryos.

(D–G) In situ hybridization using a ceruloplasmin probe with 3 dpf embryos. (D) A control embryo injected with standard morpholino control. (E–G) Examples of embryos injected with exdpf morpholino. Note comparable expression of cerulaplasmin in all embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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