FIGURE

Fig. 1

ID

ZDB-FIG-081222-6

Publication

Osborne et al., 2008 - The spinster homolog, two of hearts, is required for sphingosine 1-phosphate signaling in zebrafish

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Fig. 1

two of hearts and miles apart Mutant Phenotypes

Comparison of WT (A–C), toh mutant (D–F), and mil/s1p2 mutant (G–I) embryos.

(A, D, and G) Lateral brightfield images, anterior to the left, at 36 hpf show pericardial edema (arrow) and epidermal blisters (insets, arrowheads) in the tails of toh^sk12 (D) and mil/s1p2^m93 (G) mutants.

(B, E and H) Examination of cmlc2 expression at 19 hpf shows heart-ring formation in WT embryos (B) and a failure in precardiac-mesoderm migration in toh^s420 (E) and mil/s1p2^m93 (H) mutant embryos. Dorsal views with anterior upwards.

(C, F and I) Visualization of the anterior endoderm by Tg(-0.7her5:EGFP)^ne2067 expression at 18 hpf. In embryos injected with toh (F) and mil/s1p2 (I) MOs, numerous gaps (arrowheads) appear in the endodermal sheet, which is also irregularly shaped. The most anterior region of mil/s1p2 morphants lacks GFP⁺ endodermal cells at the midline (asterisk). Dorsal views with anterior upwards.

Expression Data

Genes:	EGFP myl7
Fish:	WT s1pr2^m93/m93 spns2^sk12/sk12 ne2067Tg ne2067Tg + MO1-spns2
Knockdown Reagent:	MO1-spns2
Anatomical Terms:	endoderm heart rudiment
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s1pr2^m93/m93 spns2^sk12/sk12 ne2067Tg + MO1-spns2
Knockdown Reagent:	MO1-spns2
Observed In:	blood circulation caudal fin endoderm endoderm heart heart rudiment
Stage Range:	14-19 somites to Prim-25

Phenotype Detail

Acknowledgments

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