Fig. 6

ChIP analysis reveals that Blimp1 binds to the promoter of the mylz2 gene. (A) The mylz2 gene promoter region contains six putative Blimp1 binding sites (red arrowheads, direction indicates orientation) based on variations of the mouse Blimp1 consensus binding sequence. Numbers indicate base pairs relative to the transcription start site (black arrow). Positions of PCR primers designed to test for Blimp1 binding are indicated (red bars, mylz2 I–IV). (B) PCR analysis of chromatin before ChIP (Input), and after immunoprecipitation with anti-HA antibodies or anti-GFP antibodies (negative control). Sequences near the β-actin1 gene served as a control for non-specific ChIP (β-actin I, II).