FIGURE

Fig. 6

ID

ZDB-FIG-081219-49

Publication

Dutton et al., 2008 - An evolutionarily conserved intronic region controls the spatiotemporal expression of the transcription factor Sox10

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Fig. 6

Functional testing of transcription factors implicated in sox10 regulation. A, B. Early zebrafish embryos (6 hpf), injected with plasmid constructs at the 1-cell stage, were divided and half subjected to a 60 minute 37°C heatshock. All were left to develop for a further 1 hour at 28.5°C before fixing. sox10 in situ hybridisation was performed to assess ectopic sox10 expression. A, A′ embryos injected with Hs:notch^ΔEmv. B, B′ embryos injected with Hs:Δβ catenin. C. Embryos of the transgenic line Tg(-4725sox10:GFP)^ba3were injected with equivalent constructs lacking GFP-coding regions. C, C′ embryos injected with hs:zsox9b. A-C no heatshock, A′-C′ with heatshock. Ectopic sox10 or gfp expression is indicated by the coloured reaction product (arrows). Note that the low level expression in B reflects the known leakiness of this heatshock promoter [71].

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.