Fig. 1

Zebrafish MHB morphogenesis occurs between 17 and 24 hpf, and requires laminin but not ventricle inflation. (A–C) Brightfield and fluorescent images and schematics of wild type (WT) MHBC formation. (D) WT embryo at 24 hpf was stained with Laminin 1 antibody (green); nuclei were stained with propidium iodide (red). Laminin lines the basal surface of the neuroepithelium. (E) Brightfield image of snk, a ventricle inflation mutant, at 24 hpf. (F) snk embryo at 24 hpf stained as in (D). (G) Brightfield image and schematic of nok, a ventricle inflation mutant, at 24 hpf. (H) nok embryo at 24 hpf stained as in as (D). (I–K) Brightfield and fluorescent images and schematics of MHBC formation in the laminin mutant, sly. (L) gup embryo at 24 hpf stained as in (D). Arrowheads indicate MHB at 21 hpf and MHBC at 24 hpf. F, forebrain; M, midbrain; H, hindbrain. Scale bars: A–C, E, G, I–K = 100 μM, D, F, H, L = 6 μM.