FIGURE

Fig. 2

ID

ZDB-FIG-080909-42

Publication

Antonellis et al., 2008 - Identification of Neural Crest and Glial Enhancers at the Mouse Sox10 Locus through Transgenesis in Zebrafish

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Fig. 2

Mouse Sox10-MCSs direct EGFP reporter expression in zebrafish embryos consistent with endogenous sox10.
A–C) Sox10-MCS1C directs reporter gene expression to the cranial ganglia (A; white arrows) and within premigratory neural crest (A; open arrowheads) at 24 hpf (A). By 72 hpf (B), signal is detected in scattered oligodendrocytes (asterisks) along the spinal column and descending Schwann cells (white arrowheads) surrounding peripheral motor neurons. Weak reporter expression is also detected along the sympathetic chain (B; white arrow). At 5 dpf (C), signal is clearly detected in the ENS (C; white arrows). D) Sox10-MCS2 directs weak EGFP expression in glial cells of the CNS (white arrowheads) and PNS (white arrow). E and F) Sox10-MCS4 directs reporter gene expression in presumptive oligodendroglial cells at 48 hpf (E; white arrowhead). By 5 dpf (F), robust signal is detected in several neural crest lineages, including Schwann cells (F; white arrowheads), sympathetic chain ganglia (open arrowheads), and the enteric nervous system (white arrows). As with endogenous sox10, EGFP expression is maintained in mature oligodendrocytes (asterisks). G and H) Sox10-MSC5 also directs signal in early oligodendroglial cells (G; white arrowheads) and sustains signal in mature oligodendrocytes (asterisks). Glial cells of the PNS (H; white arrowheads) are clearly detected at 5 dpf (H). I) In situ hybridization using GFP riboprobe detects early migrating melanoblasts (black arrows) in Sox10-MCS7 transgenic embryos at 24 hpf. J) By 5 dpf, Sox10-MCS7 directs signal to oligodendrocytes (white arrowheads) along the ventral column. K) Sox10-MCS8 directs weak reporter to the ENS (white arrows) at 5 dpf. L) at 72 hpf, sustained reporter gene expression appears in the cranial ganglia (white arrows) of Sox10-MCS9 transgenic zebrafish embryos.

Expression Data

Gene:	EGFP
Fish:	jh102Tg jh103Tg jh105Tg jh106Tg jh108Tg jh109Tg jh110Tg
Anatomical Terms:	central nervous system glial cell cranial ganglion enteric nervous system melanoblast myelinating Schwann cell neural crest oligodendrocyte peripheral nervous system glial cell spinal cord oligodendrocyte sympathetic chain ganglion
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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