FIGURE

Fig. 6

ID

ZDB-FIG-080709-24

Publication

Lin et al., 1992 - Production of germ-line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos

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Fig. 6

Southern analysis of DNA from pigmented and albino offspring of chimera 5-5-3 showing inheritance of the transgene in some pigmented offspring. Pst I digests of genomic DNA from individual fish were analyzed as described under Materials and Methods. Lane 1, albino fish DNA to which was added 50 pg of the RSV-lacZ plasmid, equivalent to approximately two copies per diploid genome; lane 2, albino fish DNA; lane 3, DNA from a heterozygous M9-1 donor transgenic fish; lanes 4-8, DNA from five individual pigmented offspring of chimera 5-5-3, three of which inherited the lacZ gene; lanes 9-11, DNA from three individual albino offspring of chimera 5-5-3, none ofthem carried the lacZ gene. kb, Kilobase(s).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA