FIGURE

Fig. 4

ID

ZDB-FIG-080620-18

Publication

Li et al., 2000 - Double-stranded RNA injection produces null phenotypes in zebrafish

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Fig. 4

The ntl phenocopy produced by microinjection of double-stranded Zf-T RNA into single-cell embryos. (A) No abnormal phenotypes were apparent subsequent to injection of Zf-T sense or antisense RNA. The injection of double-stranded control RNA also produced no apparent phenotypes. Embryos injected with double-stranded Zf-T RNA exhibited greatly reduced tails and their somites lacked the typical chevron shape of those in the wild-type embryos. The embryos shown are 5 days old (A). (B) In situ hybridizations demonstrate that 9-h embryos lack Zf-T expression following double-stranded Zf-T RNA injection into single-cell embryos, although sense RNA, antisense RNA, and doublestranded control RNA injection had little effect on Zf-T expression. Scale bars = 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 217(2), Li, Y.X., Farrell, M.J., Liu, R., Mohanty, N., and Kirby, M.L., Double-stranded RNA injection produces null phenotypes in zebrafish, 394-405, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.