FIGURE

Fig. 4

ID

ZDB-FIG-080529-41

Publication

Preger et al., 2004 - Alternative splicing generates an isoform of the human Sef gene with altered subcellular localization and specificity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

hSef-b reduces cyclin D1 levels and inhibits the Ras/MAPK pathway in FGF2-stimulated cells. Control and hSef-b inducible NIH 3T3 cells were serum starved for 24 h in the presence and absence of tet and then stimulated with FGF2 (20 ng/ml) for the indicated time periods. (A) The effect of hSef-b on the levels of cyclin D1 protein. The levels of cyclin D1 were evaluated in total cell lysates over 20 h of stimulation. Cyclin D1 and cdk proteins were analyzed by immunoblotting with anti-D1 monoclonal antibody or rabbit anti-cdk4. (B) The effect of hSef-b on FGF2-induced signaling pathways. Equal amounts of total cell lysates were analyzed by immunoblotting. The membranes were successively incubated with the indicated antibodies. (C) Activation of ERK1/2 MAPK in the control cultures. In cells transfected with control vector, there was no effect of tet removal on ERK1/2 activation. Each experiment was repeated at least twice and by using two independent clones of hSef-b inducible cells. P-ERK1/2, P-Akt, P-p38, and P-MEK1/2 are antibodies directed against the phosphorylated (P) form of the kinases.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA