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Fig. 3

ID

ZDB-FIG-080522-2

Publication

Bellipanni et al., 2000 - Expression of Otx homeodomain proteins induces cell aggregation in developing zebrafish embryos

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Fig. 3

Injected ectopically located zOtx1 RNA and endogenous zOtx1 RNA are present at similar levels in embryos showing aggregation of descendants of injected cells. Embryos were injected into 1 cell of a 16-cell embryo with approximately 1 nl of solution containing 100 μg/ml zOtx1 RNA, allowed to develop to 9 hpf, and then fixed and processed for in situ hybridization with zOtx1 probe. The embryos are shown in dorsolateral view with animal pole toward the top. Endogenous zOtx1 is expressed in a mass of cells near the animal pole (dark arrowheads), whereas cells contaning injected ectopically located zOtx1 RNA are present in aggregates located outside of the region of endogenous expresson (open arrowheads). The intensity of in situ hybridization signal in these aggregates is similar to that of the region containing endogenous zOtx1 RNA.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 223(2), Bellipanni, G., Murakami, T., Doerre, O.G., Andermann, P., and Weinberg, E.S., Expression of Otx homeodomain proteins induces cell aggregation in developing zebrafish embryos, 339-353, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.