Fig. 6

Ventral motor nerves are revealed by immunostaining for acetylated α-tubulin in embryos injected with different chondroitin sulfates. Lateral views of whole-mount preparations, anterior is to the left, dorsal to the top. Embryos are 32–34 hpf. Asterisks indicate truncated or missing ventral motor nerves, arrows abnormal side branches. (A) Injection of the chondroitin sulfate mixture (25 mg/ml) has led to truncated ventral motor nerves, illustrated are segments 12–16 on the right body side. (B) Injection of chondroitin sulfate-C (25 mg/ml) has not affected the ventral motor nerves, illustrated are segments 10–15 on the right body side. (C) Injection of chondroitin sulfate-B (25 mg/ml) has induced truncated ventral motor nerves, illustrated in this overview are segments 8–16 on the left body side. (D) Injection of chondroitin sulfate-B (5 mg/ml) has induced truncated and branched ventral motor nerves, illustrated are segments 11–16. Magnification bar: 50 μm for (A, B, D), 100 μm for (C).