FIGURE

Fig. 9

ID

ZDB-FIG-080506-47

Publication

Faucourt et al., 2001 - The pitx2 homeobox protein is required early for endoderm formation and nodal signaling

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Fig. 9

Change in the fate of vegetal blastomeres injected with low doses of EnR-pitx2 and reversal by wild-type pitx2 mRNA. Embryos were injected into one ventral vegetal blastomere at the eight-cell stage with β-Gal RNA alone (A, D), β-Gal plus 75 pg EnR-pitx2 RNA (B, E), β-Gal plus 200 pg wt pitx2 RNA (C), or β-Gal plus 75 pg EnR-pitx2 RNA plus 200 pg of wt pitx2 RNA (F) and processed for detection of the lineage label around stage 34. The shift in position of the progeny of the injected cells caused by EnR-pitx2 is reversed by wild-type pitx2. (D, E) Histological sections from equivalent posterior positions through embryos injected with β-Gal RNA (D) or with EnR-pitx2 RNA (E). Note that while in the control embryo β-Gal-positive cells populate mesodermal and endodermal structures, β-Gal-positive cells are absent from the endoderm in the EnR-pitx2-injected embryo.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 229(2), Faucourt, M., Houliston, E., Besnardeau, L., Kimelman, D., and Lepage, T., The pitx2 homeobox protein is required early for endoderm formation and nodal signaling, 287-306, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.