FIGURE

Fig. S4

ID

ZDB-FIG-080501-14

Publication

Choi et al., 2008 - Members of the miRNA-200 Family Regulate Olfactory Neurogenesis

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Fig. S4

Anti-Sense Morpholino Oligos Specifically and Efficiently Knock Down Expression of Their Cognate Zebrafish miR-200 Targets
(A) Sequence alignments of synthesized antisense morpholino oligos directed against various miR-200 family members and their corresponding miR-200 family members. miR-141 MO is predicted to specifically hybridize to miR-141 and miR-200a, miR-200b MO is predicted to specifically hybridize to miR-200b and miR-200c and miR-429 MO is predicted to specifically hybridize to miR-420.
(B) One-cell embryos were injected with 4 ng of either miR-141 MO, miR-200b MO or miR-429 MO and systematically assayed for expression of miR-200 family members at 48 hpf indicating that the predictions made in (A) are valid.
(C) Wild type and fish injected with various morpholinos at 48 hpf are morphologically indistinguishable from each other.

Expression Data

Genes:	dre-mir-429 mir141 mir200a mir200b mir200c
Fish:	WT WT + MO1-dre-mir-429 WT + MO1-mir141,mir200a WT + MO1-mir200b,mir200c
Knockdown Reagents:	MO1-dre-mir-429 MO1-mir141,mir200a MO1-mir200b,mir200c
Anatomical Term:	peripheral olfactory organ
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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