Fig. 10

Severity of the puma mutant phenotype depends on temperature and growth rate. (A) Wild-type individuals reared at 24°C develop typical wild-type melanophore stripes. (B) Wild-type individuals reared at 33°C also develop typical wild-type stripes. (C, D) The puma mutant phenotype is temperature-dependent. (C) At 24°C, melanophore densities and patterns approach that seen in wild-type individuals. (D) At 33°C, however, a severe melanophore deficiency and pattern defect is observed. (C–F) The severity of the puma mutant phenotype depends on individual growth rate as well as temperature. LG, low growth. HG, high growth. (C, D) puma mutants reared under conditions favoring a low growth rate (LG) exhibit markedly different phenotypes depending on the temperature they experience during development. (E, F) puma mutants reared under conditions favoring a high growth rate (HG) exhibit similar phenotypes across temperatures, since even individuals reared at 24°C exhibit a severe melanophore deficiency. In contrast to puma mutants, phenotypes of wild-type fish did not differ dramatically given different growth rates (e.g., A, high growth; B, low growth). In all images, the region shown is immediately dorsal to the anal fin.