Staining of actin (phalloidin staining, red) and acetylated tubulin (green) in hair cell patches in the inner ear showed defects in tmie morphants. A,B: Double staining of actin (stereocilia) and tubulin (kinocilia) of the three cristae (arrowheads). C,D: Double staining of stereocilia and cristae in the maculae (arrows). E,F: Actin staining showing the epithelial pillars (ep), which form hubs of the developing semicircular canals. Arrows indicate the junction of the ventral bulge (b) and ventral projection (p). In the control ear, the junction has formed and fused (E), but in the tmie MO-treated fish, there is still a gap between the bulge and the projection. G-J: Phalloidin staining of the anterior macula (arrowhead) at 5 dpf (G, H) and 6 dpf (I, J). The patch is smaller in tmie morphants, especially at 6 dpf. K: Numbers of hair cells labeled by phalloidin at 4 dpf (n = 5 for wt, and n = 4 for MOstmie), and as illustrated in G-H at 5 dpf (n = 3 for wt, n = 6 for control, and n = 13 for MOstmie) and 6 dpf (n = 2 for wt, n = 2 for control, and n = 4 for MOstmie). L-O: Acetylated tubulin staining of the posterior macula.
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