FIGURE

Fig. 2

ID

ZDB-FIG-071112-37

Publication

Kwan et al., 2007 - The Tol2kit: A multisite gateway-based construction kit for Tol2 transposon transgenesis constructs

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Fig. 2

Validation of reporters and internal ribosome entry sequence (IRES) constructs. Embryos shown were injected at the one-cell stage with expression constructs made with the pISce-Dest destination vector, then mounted at 24 hours postfertilization (hpf) for confocal microscopy of the trunk. All plasmids tested generate functional bicistronic messages, as demonstrated by the presence of both mCherry and EGFP fluorescence. A-A″:bactin2: nlsmCherry-IRES-EGFPCAAXpA. B-B″:h2afx: nlsmCherry-IRES-EGFPCAAXpA. C-C″:bactin2: mCherry-IRES-EGFPCAAXpA. D-D″:bactin2: nlsmCherry-IRES-EGFPpA. E-E″:bactin2: mCherryCAAX-IRES-nlsEGFPpA. F:bactin2: nlsEGFP-IRES-EGFPCAAXpA. G:bactin2: EGFPCAAX-IRES-nlsEGFPpA. Scale bar = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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