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Fig. 3

ID

ZDB-FIG-070927-32

Publication

Zhu et al., 2005 - Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish

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Fig. 3

Transplantation of primitive progenitors into vlad tepes (vlt) recipients. (A) The EGFP+ population from 10- to 12-somite LGb; Tg(gata1:DsRed) transgenic embryos was isolated by FACS to 90% purity and transplanted into 48 hpf vlt embryos. (B) One day after transplantation, circulating donor-derived cells were identified by DsRed and EGFP fluorescence. By 3 days post transplantation, most circulating cells were DsRed⁺, suggesting that the EGFP+ donor progenitors had differentiated into Gata1⁺ erythrocytes (arrow). One month after transplantation, each of the 8 survivors carried approximately 10–200 DsRed+ circulating cells (arrows). At 1 month, we also observed EGFP expression in the trunk vasculature (arrowheads) in 2 of 8 recipients. A table summarizing the results is shown in C.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 281(2), Zhu, H., Traver, D., Davidson, A.J., Dibiase, A., Thisse, C., Thisse, B., Nimer, S., and Zon, L.I., Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish, 256-269, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.