FIGURE

Fig. 9

ID

ZDB-FIG-070918-50

Publication

Ho et al., 2006 - Inhibitor-resistant type I receptors reveal specific requirements for TGF-beta signaling in vivo

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Fig. 9

Alk4 but not Alk7 can rescue signaling during gastrulation (A) Alk4* and Alk7*-injected animal caps were treated with 100 μM SB-431542 and p-Smad2 signaling was assessed by Western blot at stage 10.5. (B) Embryos injected with different amounts of xAlk4-HA or rAlk7-HA were treated with 100 μM SB-431542 at stage 6 and harvested for Western blotting at stage 10+. All mRNA levels were equalized to 6 ng total with eGFP. Embryos injected with (C, D) 3 ng xAlk4-HA S275M, (E, F) 3 ng xAlk4-HA WT, (G, H) 4 ng rAlk7-HA S270M, or (I, J) 4 ng rAlk7-HA WT were treated as above and photographed at stage 10+. Note rescue of blastopore lip formation in Alk4 S275M embryo (red arrow in panel B). Embryos are photographed with the vegetal pole showing, dorsal to the upper left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 295(2), Ho, D.M., Chan, J., Bayliss, P., and Whitman, M., Inhibitor-resistant type I receptors reveal specific requirements for TGF-beta signaling in vivo, 730-742, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.