Fig. 8
Loss of PGCs in Stau disrupted embryos is rescued by co-injection of Drosophila staufen. GFPnos1 expression (A–H, Q) or vasa expression (I–P, R) in embryos (24 hpf) injected with GST protein + lacZ RNA (A, I), GST protein + Drosophila melanogaster staufen RNA (B, J), Stau2 RBD5 peptide + lacZ RNA (C, K), Stau2 RBD5 peptide + Drosophila melanogaster staufen RNA (D, L), control morpholinos + lacZ RNA (E, M), control morpholino + Drosophila melanogaster staufen RNA (F, N), Staufen2 morpholino2 + lacZ RNA (G, O), and staufen2 morpholino2 + Drosophila melanogaster staufen RNA (H, P). Dotted boxes in panels A–P outline the areas shown at higher magnification in the insets. Lateral views, scale bar, 100 μm. (Q) % embryos with GFPnos expression in PGCs, (R) % embryos with vasa RNA expression at 24 hpf. |
Reprinted from Developmental Biology, 292(2), Ramasamy, S., Wang, H., Quach, H.N., and Sampath, K., Zebrafish Staufen1 and Staufen2 are required for the survival and migration of primordial germ cells, 393-406, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.