FIGURE

Fig. 7

ID

ZDB-FIG-070914-68

Publication

Steffen et al., 2007 - The zebrafish runzel muscular dystrophy is linked to the titin gene

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

The ruz dystrophy shows increased calpain-3 and loss of sarcomeric MuRF2 expression. (A) Western blot analysis of 6.5 dpf wild-type and ruz mutants shows a mild increase in calpain-3. Actin expression is used as a loading control. (B, C) Indirect immunofluorescence using MuRF2 antibodies (red) was performed on longitudinally sectioned skeletal muscle of 6.5 dpf fish. Nuclei were stained with DAPI (blue). (B) In wild-type fish, MuRF2 is localized to both the sarcomere (striations) and nucleus (arrow). (C) MuRF2 is lost at the sarcomere in ruz mutants but no significant increase in nuclear localization is detected.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ruz^tk258/tk258 (TL)
Observed In:	skeletal muscle cell sarcomere
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 309(2), Steffen, L.S., Guyon, J.R., Vogel, E.D., Howell, M.H., Zhou, Y., Weber, G.J., Zon, L.I., and Kunkel, L.M., The zebrafish runzel muscular dystrophy is linked to the titin gene, 180-192, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.