Fig. 1

Cardiac-specific expression of zlrrc10. (A) The genomic structure of zlrrc10. The full-length zlrrc10 cDNA consists of two exons. The size of exons, intron and the 5′ and 3′ UTR regions are shown in bp. The amino acid coding regions are represented by the hatched box. (B) An alignment of zebrafish, mouse and human LRRC10 amino acid sequences. Identical residues between all three species are shaded black, and the residues with similar characteristics are shaded gray. The conserved seven LRR motifs are numbered. (C) Cardiac-specific expression of lrrc10 in zebrafish embryos. Zebrafish embryos at 1 dpf (a–c) and 2 dpf (d–f) were subjected to whole-mount in situ hybridization using digoxigenin labeled zlrrc10 antisense cRNA probes (a, d). cmlc2 antisense cRNA probe (b and e) was used for positive control to visualize the heart. Negative control was hybridized with sense zlrrc10 probe (c and f). Arrows indicate the linear heart region that expresses zlrrc10 and cmlc2. A and V indicate the atrium and ventricle, respectively. (D) Cardiac-specific expression of zlrrc10 in adult zebrafish. RT-PCR analysis was performed using total RNA isolated from three different parts of adult zebrafish as indicated. The zlrrc10 PCR products of 581 bp were visualized by agarose gel electrophoresis. The plasmid pCRII-TOPO-zlrrc10 was used as positive control for the amplification reactions. RT-PCR of β-actin was performed as a loading control.