In situ hybridization reveals neural expression of scn5Laa. A: At 24 hours postfertilization (hpf), scn5Laa mRNA is detected in regions associated with the developing heart tube and pharynx (arrows). B: Under the same conditions, the sense probe does not reveal a signal. C: At 48 hpf, scn5Laa expression shifts to more anterior and dorsal regions of the embryo (e.g., head muscle, arrowhead). D,E: At 60 hpf, scn5Laa expression appears in rostral regions of the central nervous system as well as the developing pectoral fin (arrow). Scale bars = 250 μm in A (for A-E). as, antisense probe; s, sense probe.
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