Fig. 4
- ID
- ZDB-FIG-060605-26
- Publication
- Pyati et al., 2006 - Sustained Bmp signaling is essential for cloaca development in zebrafish
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Transgenic embryos do not develop a functional cloaca. (A) Overview of the rhodamine gut injection assay used to assess cloacal function in wild-type and transgenic embryos. (B,C) Injected wild-type embryos secrete the rhodamine dextran from their cloaca within minutes. (D,E) Transgenic embryos develop a buildup of rhodamine dextran at the end of their gut, and they do not excrete the dye into the media. B,D are brightfield views; C,E are brightfield/fluorescent merged images. Brackets in B-E indicate the gut terminus. |