FIGURE

Fig. 7

ID

ZDB-FIG-060207-2

Publication

Hou et al., 2006 - Recapitulation of human betaB1-crystallin promoter activity in transgenic zebrafish

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Fig. 7

Maf binding cis-element is crucial for the upstream promoter activity of the human βB1-crystallin gene. Top: A point mutation was introduced in the single Maf binding site of the human $beta;B1-crystallin promoter. The mutated and wild-type upstream promoter -2,966/+61-bp fragments were analyzed in zebrafish by transient transgenic assay. Mutation of the Maf binding site severely impeded the human βB1-crystallin promoter function as reflected by the decrease in percentage of injected embryos that express EGFP in the lens. A-D: With the wild-type construct, intensive expression of EGFP is shown in the whole lens of most embryos (A), and in a few embryos with mosaic patterns (B-D). E-H: Embryos injected with the mutated construct show highly mosaic patterns and the reporter gene, EGFP, is faintly expressed.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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