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In situ hybridizations of eve1 in wild-type and mutant embryos (whole mounts). Wild types are on the left of each picture and homozygous mutants on the right. Anterior poles at the top. Dorsal sides on the right. (A,B) spadetail mutation. (A) 12.5 h embryos (5- somite stage for the wild type). Beginning of abnormal tail bud swelling can be seen at the bottom of the embryo on the right. (B) 22 h embryo. In spt homozygous mutants, eve1 staining covers the whole swollen tail bud and anterior extension is longer than in wild-types. (C,D) no tail mutation. (C) 5-somite stage (12.5 h). No signal is observed in the mutant embryo. (D) 24 h embryos. Signal begins to fade in the wild-type embryo but is completely absent in the homozygous mutant.
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