FIGURE

Fig. 4

ID

ZDB-FIG-050926-4

Publication

Thummel et al., 2005 - Cre-mediated site-specific recombination in zebrafish embryos

Other Figures

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Fig. 4

Analysis of HSP70-EGFP-cre transgenic line. A: Southern transfer analysis of HSP70-EGFP-cre transgenic line. B: Reverse transcriptase-polymerase chain reaction analysis of HSP70-EGFP-cre transgenic line. "H20" denotes no DNA control reaction. "+" and "-" denote addition and absence of reverse transcriptase, respectively. C: Western blot detection of EGFP-Cre fusion protein. Lane 1: un-induced wild-type fish; lane 2: un-induced HSP70-EGFP-cre Tg/+ fish; lane 3: wild-type fish heat shock induced for 1 hr at 37°C; lane 4: HSP70-EGFP-cre Tg/+ fish heat shock induced for 1 hr at 37°C; lane 5: wild-type fish heat shock induced for 1 hr at 39°C; lane 6; HSP70-EGFP-cre Tg/+ fish heat shock induced for 1 hr at 39°C; lane 7: wild-type fish heat shock induced for 5 min at 42°C; lane 8: HSP70-EGFP-cre Tg/+ fish heat shock induced for 5 min at 42°C.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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