ZFIN ID: ZDB-ETCONSTRCT-120604-1
Enhancer Trap Construct Name: Et(GAL4-VP16,myl7:GFP)
Sequence Ontology ID :
Synonyms: Et(Gal4-VP16), Et(bp:Gal4VP16) (1), Et(Gal4-VP16,myl7:GFP)

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Note: This enhancer trapping construct is based on pDestTol2CG3, a multisite-Gateway-based plasmid. It contains a minimal adenovirus E1b promoter driving Gal4VP16 (Gal4 DNA-binding domain fused to the VP16 transcriptional activation domain) followed by the late SV40 polyA signal sequence. Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving EGFP followed by the SV40 late polyA signal sequence; this marks the heart with green fluorescence. The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions. The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).

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CONSTRUCT COMPONENTS
Regulatory Regions: myl7 (1)
Coding Sequences: GAL4 (1), GFP (1)
VP16 (1)
GENOMIC FEATURES THAT UTILIZE Et(GAL4-VP16,myl7:GFP)
View all genomic features that utilize Et(GAL4-VP16,myl7:GFP)
TRANSGENICS THAT UTILIZE Et(GAL4-VP16,myl7:GFP)
View all transgenics that utilize Et(GAL4-VP16,myl7:GFP)
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:KC800697
OTHER Et(GAL4-VP16,myl7:GFP) ENHANCER TRAP CONSTRUCT PAGESNo links to external sites
CITATIONS (11)