Enhancer Trap Construct

Et(GAL4-VP16,myl7:GFP)

ID
ZDB-ETCONSTRCT-120604-1
Name
Et(GAL4-VP16,myl7:GFP)
Previous Names
  • Et(Gal4-VP16)
  • Et(bp:Gal4VP16) (1)
  • Et(Gal4-VP16,myl7:GFP)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
Contains Sequences
None
Note
This enhancer trapping construct is based on pDestTol2CG3, a multisite-Gateway-based plasmid. It contains a minimal adenovirus E1b promoter driving Gal4VP16 (Gal4 DNA-binding domain fused to the VP16 transcriptional activation domain) followed by the late SV40 polyA signal sequence. Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving EGFP followed by the SV40 late polyA signal sequence; this marks the heart with green fluorescence. The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions. The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).
Plasmid Map
Genomic Features
That Utilize Et(GAL4-VP16,myl7:GFP) Construct
Transgenics
That Utilize Et(GAL4-VP16,myl7:GFP) Construct
Fish Affected Genomic Regions Phenotype Gene Expression
c264Tg/+; zc1000aEt/+
c264Tg/+; zc1004aEt/+
c264Tg/+; zc1008aEt/+
c264Tg/+; zc1009aEt/+
c264Tg/+; zc1012aEt/+
c264Tg/+; zc1013aEt/+
c264Tg/+; zc1015aEt/+
c264Tg/+; zc1016aEt/+
c264Tg/+; zc1016cEt/+
c264Tg/+; zc1017aEt/+
c264Tg/+; zc1018aEt/+
c264Tg; zc1000aEt
8 figures with image from Hutcheson et al., 2013
c264Tg; zc1002aEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1003aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1004aEt
10 figures with image from 2 publications
c264Tg; zc1006aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1008aEt
6 figures with image from Hutcheson et al., 2013
c264Tg; zc1009aEt
12 figures with image from 2 publications
c264Tg; zc1010aEt
9 figures with image from Hutcheson et al., 2014
c264Tg; zc1012aEt
10 figures with image from Hutcheson et al., 2013
c264Tg; zc1013aEt
8 figures with image from Hutcheson et al., 2013
c264Tg; zc1015aEt
6 figures with image from Hutcheson et al., 2013
c264Tg; zc1016aEt
11 figures with image from Hutcheson et al., 2013
c264Tg; zc1016cEt
9 figures with image from 2 publications
c264Tg; zc1016dEt
3 figures with image from Hutcheson et al., 2014
c264Tg; zc1017aEt
6 figures with image from Hutcheson et al., 2013
c264Tg; zc1018aEt
10 figures with image from Hutcheson et al., 2013
c264Tg; zc1019aEt
7 figures with image from 2 publications
c264Tg; zc1019bEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1020aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1020bEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1021aEt
6 figures with image from Hutcheson et al., 2014
c264Tg; zc1022aEt
4 figures with image from Hutcheson et al., 2014
c264Tg; zc1022bEt
7 figures with image from 2 publications
c264Tg; zc1023aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1023bEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1023dEt
6 figures with image from Hutcheson et al., 2014
c264Tg; zc1024aEt
5 figures with image from 2 publications
c264Tg; zc1025aEt
10 figures with image from 2 publications
c264Tg; zc1025bEt
10 figures with image from 2 publications
c264Tg; zc1025dEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1026aEt
6 figures with image from Hutcheson et al., 2014
c264Tg; zc1028aEt
c264Tg; zc1029eEt
5 figures with image from 2 publications
c264Tg; zc1030aEt
8 figures with image from 2 publications
c264Tg; zc1031aEt
16 figures with image from 2 publications
c264Tg; zc1032aEt
9 figures with image from 2 publications
c264Tg; zc1033aEt
11 figures with image from 2 publications
c264Tg; zc1034aEt
9 figures with image from 2 publications
c264Tg; zc1035aEt
9 figures with image from 2 publications
c264Tg; zc1036aEt
9 figures with image from 2 publications
c264Tg; zc1037aEt
9 figures with image from 2 publications
c264Tg; zc1037bEt
12 figures with image from 2 publications
c264Tg; zc1037cEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1038aEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1039aEt
13 figures with image from Hutcheson et al., 2014
c264Tg; zc1039bEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1040aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1040bEt
9 figures with image from Hutcheson et al., 2014
c264Tg; zc1041aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1042aEt
14 figures with image from 2 publications
c264Tg; zc1044aEt
10 figures with image from Hutcheson et al., 2014
c264Tg; zc1046aEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1047aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1048aEt
12 figures with image from Hutcheson et al., 2014
c264Tg; zc1049aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1050aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1051aEt
14 figures with image from 2 publications
c264Tg; zc1052aEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1052bEt
6 figures with image from Hutcheson et al., 2014
c264Tg; zc1053aEt
9 figures with image from 2 publications
c264Tg; zc1054aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1055aEt
14 figures with image from Hutcheson et al., 2014
c264Tg; zc1055bEt
7 figures with image from Hutcheson et al., 2014
c264Tg; zc1056aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1057aEt
6 figures with image from Hutcheson et al., 2014
c264Tg; zc1059aEt
c264Tg; zc1059bEt
c264Tg; zc1060aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1062aEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1062bEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1063aEt
11 figures with image from 2 publications
c264Tg; zc1063bEt
10 figures with image from Hutcheson et al., 2014
c264Tg; zc1064aEt
c264Tg; zc1065aEt
9 figures with image from Hutcheson et al., 2014
c264Tg; zc1066aEt
Fig. 5 with image from Otsuna et al., 2015 10 figures with image from 2 publications
c264Tg; zc1067aEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1068aEt
5 figures with image from Hutcheson et al., 2014
c264Tg; zc1069aEt
8 figures with image from Hutcheson et al., 2014
c264Tg; zc1070aEt
9 figures with image from Hutcheson et al., 2014
c264Tg; zc1071aEt
10 figures with image from Hutcheson et al., 2014
c264Tg; zc1072aEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1073aEt
c264Tg; zc1074bEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1075aEt
2 figures with image from Hutcheson et al., 2014
c264Tg; zc1076aEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1077aEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1085aEt
11 figures with image from Hutcheson et al., 2014
c264Tg; zc1086aEt
9 figures with image from Hutcheson et al., 2014
c264Tg; zc1094aEt
jh17Tg; w32Tg; zc1066aEt
Fig. 4 with image from Wang et al., 2012 Fig. 4 with image from Wang et al., 2012
jh17Tg; w34Tg; zc1066aEt
Fig. 4 with image from Wang et al., 2012 Fig. 4 with image from Wang et al., 2012
jh17Tg; zc1066aEt
Fig. 4 with image from Wang et al., 2012
nkuasgfp1aTg/+; zc1007aEt/+
nkuasgfp1aTg/+; zc1011aEt/+
nkuasgfp1aTg; zc1007aEt
11 figures with image from Hutcheson et al., 2013
nkuasgfp1aTg; zc1011aEt
5 figures with image from Hutcheson et al., 2013
nkuasgfp1aTg; zc1022bEt
nkuasgfp1aTg; zc1033aEt
nkuasgfp1aTg; zc1036aEt
nkuasgfp1aTg; zc1037cEt
nkuasgfp1aTg; zc1040aEt
nkuasgfp1aTg; zc1060aEt
s1999tTg; zc1044aEt + MO1-piezo1
Fig. S10 from Eisenhoffer et al., 2012 Fig. S10 from Eisenhoffer et al., 2012
s1999tTg; zc1052bEt
s1999tTg; zc1076aEt
s1999tTg; zc1077aEt
zc1002aEt
zc1002aEt/+
zc1003aEt
zc1003aEt/+
zc1006aEt
zc1006aEt/+
zc1010aEt
zc1010aEt/+
zc1016dEt
zc1016dEt/+
zc1021aEt
zc1038aEt
zc1046aEt
zc1062aEt
zc1065aEt
zc1094aEt
Sequence Information
Other Construct Pages
None
Citations