CRISPR

CRISPR2-wrn

ID

ZDB-CRISPR-260408-8

Name

CRISPR2-wrn

Previous Names

None

Target

wrn (1)

Sequence

5' - TGTGTGGTGGCCACA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GTG" at the 3' end.

Genome Resources

None

Target Location

View all 5 target locations

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
agu26	wrn

Expression

Gene expression in Wild Types + CRISPR2-wrn

No data available

Phenotype

Phenotype resulting from CRISPR2-wrn

No data available

Phenotype of all Fish created by or utilizing CRISPR2-wrn

Phenotype	Fish	Conditions	Figures
whole organism glycogen decreased amount, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	standard conditions	Figure 5 from Ujibe et al., 2026
whole organism viability, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	control	Figure 1 , Figure 5 from Ujibe et al., 2026
whole organism glucose decreased amount, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	standard conditions	Figure 5 from Ujibe et al., 2026
whole organism viability, ameliorated	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	increased food availability	Figure 5 from Ujibe et al., 2026
whole organism decreased length, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	standard conditions	Figure 1 from Ujibe et al., 2026
whole organism lipid decreased amount, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	standard conditions	Figure 5 from Ujibe et al., 2026
whole organism dead, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9	limited food availability	Figure 5 from Ujibe et al., 2026
pancreas Ab12-h2afx labeling increased distribution, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9; jh1Tg	standard conditions	Figure 4 from Ujibe et al., 2026
exocrine pancreas EGFP expression spatial pattern, ameliorated	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9; jh1Tg	increased food availability	Figure 5 from Ujibe et al., 2026
intestine Ab12-h2afx labeling increased distribution, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9; jh1Tg	standard conditions	Figure 4 from Ujibe et al., 2026
pancreas apoptotic process increased process quality, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9; jh1Tg	standard conditions	Figure 4 from Ujibe et al., 2026
exocrine pancreas EGFP expression decreased distribution, abnormal	mitfa^w2/w2; wrn^agu26/agu26; mpv17^a9/a9; jh1Tg	control	Figure 3 , Figure 4 , Figure 5 from Ujibe et al., 2026

Citations