CRISPR

CRISPR27-tp53

ID

ZDB-CRISPR-250905-2

Name

CRISPR27-tp53

Previous Names

None

Target

tp53 (1)

Sequence

5' - TGCAGTGGCAGCGAATGAAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sou101	tp53

Expression

Gene expression in Wild Types + CRISPR27-tp53

No data available

Phenotype

Phenotype resulting from CRISPR27-tp53

No data available

Phenotype of all Fish created by or utilizing CRISPR27-tp53

Phenotype	Fish	Conditions	Figures
female organism gill cp expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
female organism gill ggt1l2.2 expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
gill glutathione peroxidase activity decreased process quality, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
female organism gill tp53 expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 3 from Li et al., 2025
gill reactive oxygen species increased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
female organism gill hbegfb expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
female organism gill tp73 expression increased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 3 from Li et al., 2025
whole organism tp53 expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	standard conditions	Fig. 1 from Li et al., 2025
female organism viability, exacerbated	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 3 from Li et al., 2025
female organism swimming behavior increased process quality, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 3 from Li et al., 2025
female organism gill ccs expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
female organism gill tp63 expression increased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 3 from Li et al., 2025
female organism gill zfand6 expression decreased amount, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025
gill superoxide dismutase activity decreased process quality, abnormal	tp53^{sou101/sou101} (AB)	cold exposure	Fig. 4 from Li et al., 2025

Citations