CRISPR

CRISPR2-abca4b

ID

ZDB-CRISPR-250502-12

Name

CRISPR2-abca4b

Previous Names

None

Target

abca4b (1)

Sequence

5' - GAATCCCTTGGATCCAAGGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added. The PAM site was 'AGG' at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf4361	abca4b

Expression

Gene expression in Wild Types + CRISPR2-abca4b

No data available

Phenotype

Phenotype resulting from CRISPR2-abca4b

No data available

Phenotype of all Fish created by or utilizing CRISPR2-abca4b

Phenotype	Fish	Conditions	Figures
long single cone cell cone photoreceptor outer segment tubular, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment erose, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
long double cone cell cone photoreceptor outer segment elongated, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment tubular, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment blunt, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment elongated, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment dysplastic, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short double cone cell phagocytic vesicle decreased amount, abnormal	abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
long double cone cell cone photoreceptor outer segment morphology, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. from Willoughby et al., 2024
long double cone cell cone photoreceptor outer segment elongated, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
short single cone cell phagocytic vesicle increased amount, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment morphology, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment decreased width, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment erose, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
long single cone cell phagocytic vesicle decreased amount, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment organization quality, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
long double cone cell phagocytic vesicle decreased amount, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. from Willoughby et al., 2024
short single cone cell phagocytic vesicle mislocalised, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment serrated, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment elongated, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment dysplastic, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment blunt, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
short double cone cell phagocytic vesicle decreased amount, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment increased length, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. from Willoughby et al., 2024
short double cone cell cone photoreceptor disc membrane misaligned with short double cone cell cone photoreceptor disc membrane, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment tubular, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
short double cone cell cone photoreceptor disc membrane organization quality, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment tubular, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. , Fig. 6. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment morphology, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 6. , Fig. 7. from Willoughby et al., 2024
retinal cone cell phosphatidyl-L-serine mislocalised, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 8. from Willoughby et al., 2024
long single cone cell cone photoreceptor disc membrane organization quality, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment decreased diameter, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment degenerate, abnormal	abca4a^{zf4359/zf4359}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 7. from Willoughby et al., 2024
long double cone cell cone photoreceptor outer segment elongated, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment erose, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short double cone cell cone photoreceptor outer segment dysplastic, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment blunt, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short double cone cell phagocytic vesicle decreased amount, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment tubular, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
long single cone cell cone photoreceptor outer segment tubular, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024
short single cone cell cone photoreceptor outer segment elongated, abnormal	abca4a^{zf4360/zf4360}; abca4b^{zf4361/zf4361}; slc45a2^b4/b4	standard conditions	Fig. 3. , Fig. 4. from Willoughby et al., 2024

Citations