CRISPR

CRISPR1-znf281b

ID
ZDB-CRISPR-250325-1
Name
CRISPR1-znf281b
Previous Names
None
Target
Sequence
5' - GTATGGGTATTAAACAGGAGAAG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
This is a Cas13d CRISPR.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
No data available
Expression
Gene expression in Wild Types + CRISPR1-znf281b
No data available
Phenotype
Phenotype resulting from CRISPR1-znf281b
Phenotype Fish Figures
gastrulation delayed, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
post-vent region deformed, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
presumptive endoderm sox32 expression decreased amount, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
presumptive mesoderm pcdh8 expression decreased amount, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
Spemann organizer formation at the embryonic shield delayed, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
whole organism znf281b expression decreased amount, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
whole organism deformed, abnormal AB/TU + CRISPR1-znf281b Fig. 5 with image from da Silva Pescador et al., 2024
Phenotype of all Fish created by or utilizing CRISPR1-znf281b
Phenotype Fish Conditions Figures
whole organism znf281b expression decreased amount, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
whole organism deformed, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
post-vent region deformed, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
presumptive mesoderm pcdh8 expression decreased amount, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
Spemann organizer formation at the embryonic shield delayed, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
presumptive endoderm sox32 expression decreased amount, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
gastrulation delayed, abnormal AB/TU + CRISPR1-znf281b control Fig. 5 with image from da Silva Pescador et al., 2024
Citations
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