CRISPR

CRISPR6-spast

ID

ZDB-CRISPR-250305-34

Name

CRISPR6-spast

Previous Names

None

Target

Sequence

5' - GCCACGATCTCGTTGAGGATG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added. The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR6-spast

No data available

Phenotype

Phenotype resulting from CRISPR6-spast

Phenotype	Fish	Figures
primary motor neuron axon branchiness, abnormal	ml2Tg + CRISPR6-spast (AB)	Fig. S5 from Luo et al., 2024
primary motor neuron axon GFP expression decreased distribution, abnormal	ml2Tg + CRISPR6-spast (AB)	Fig. S5 from Luo et al., 2024
primary motor neuron axon morphology, abnormal	ml2Tg + CRISPR6-spast (AB)	Fig. S5 from Luo et al., 2024
primary motor neuron axon GFP expression spatial pattern, abnormal	ml2Tg + CRISPR6-spast (AB)	Fig. S5 from Luo et al., 2024

Phenotype of all Fish created by or utilizing CRISPR6-spast

Phenotype	Fish	Conditions	Figures
primary motor neuron axon branchiness, abnormal	ml2Tg + CRISPR6-spast (AB)	control	Fig. S5 from Luo et al., 2024
primary motor neuron axon GFP expression decreased distribution, abnormal	ml2Tg + CRISPR6-spast (AB)	control	Fig. S5 from Luo et al., 2024
primary motor neuron axon morphology, abnormal	ml2Tg + CRISPR6-spast (AB)	control	Fig. S5 from Luo et al., 2024
primary motor neuron axon GFP expression spatial pattern, abnormal	ml2Tg + CRISPR6-spast (AB)	control	Fig. S5 from Luo et al., 2024

Citations