CRISPR

CRISPR2-akt1

ID
ZDB-CRISPR-250221-3
Name
CRISPR2-akt1
Previous Names
None
Target
Sequence
5' - GGGAAGGTGATTCTGGTGA - 3'
ZFIN BLAST NCBI BLAST Ensembl
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ya348 akt1
Expression
Gene expression in Wild Types + CRISPR2-akt1
No data available
Phenotype
Phenotype resulting from CRISPR2-akt1
No data available
Phenotype of all Fish created by or utilizing CRISPR2-akt1
Phenotype Fish Conditions Figures
posterior cardinal vein flt4 expression increased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
artery endothelial cell fate specification decreased process quality, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 4 with image from Zhou et al., 2024
artery efnb2a expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 4 with image from Zhou et al., 2024
intersegmental vessel dlc expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 5 with image from Zhou et al., 2024
dorsal aorta dlc expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image,  Fig. 5 with image from Zhou et al., 2024
mandibular arch skeleton malformed, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
whole organism akt3a expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
whole organism akt1 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
artery Notch signaling pathway decreased process quality, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 5 with image from Zhou et al., 2024
whole organism ab7-akt labeling decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
dorsal aorta notch3 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
posterior cardinal vein fli1 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
dorsal aorta fli1 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
whole organism akt3b expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
posterior cardinal vein notch3 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
dorsal aorta decreased width, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
whole organism Ab15-akt labeling decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
whole organism viability, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
dorsal aorta efnb2a expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
whole organism akt2 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
artery vegfc expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 4 with image from Zhou et al., 2024
pericardium edematous, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 1 with image from Zhou et al., 2024
posterior cardinal vein dlc expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
posterior cardinal vein efnb2a expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
artery notch3 expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
dorsal aorta flt4 expression increased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 3 with image from Zhou et al., 2024
artery dlc expression decreased amount, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349 standard conditions Fig. 4 with image,  Fig. 5 with image from Zhou et al., 2024
posterior cardinal vein YFP expression spatial pattern, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
intersegmental vessel YFP expression spatial pattern, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
dorsal aorta mCherry expression spatial pattern, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
intersegmental vessel mCherry expression spatial pattern, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
dorsal aorta decreased width, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
posterior cardinal vein mCherry expression spatial pattern, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
posterior cardinal vein decreased width, abnormal akt1ya348/ya348; akt3aya350/ya350; akt3bya351/ya351; akt2ya349/ya349; hu4881Tg/hu4881Tg; s916Tg/s916Tg standard conditions Fig. 2 with image from Zhou et al., 2024
Citations
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