CRISPR

CRISPR3-pmela

ID

ZDB-CRISPR-240906-7

Name

CRISPR3-pmela

Previous Names

None

Target

Sequence

5' - TGGAGCTTCGACTGCAGGTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ua5030	pmela

Expression

Gene expression in Wild Types + CRISPR3-pmela

No data available

Phenotype

Phenotype resulting from CRISPR3-pmela

No data available

Phenotype of all Fish created by or utilizing CRISPR3-pmela

Phenotype	Fish	Conditions	Figures
eye pmela expression absent, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Fig. S2 from Hodges et al., 2023
retina melanosome decreased diameter, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Figure 5 from Hodges et al., 2023
whole organism pmela expression decreased amount, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Fig. S2, Figure 2 from Hodges et al., 2023
integument decreased pigmentation, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Figure 2 , Figure 3 from Hodges et al., 2023
retina Melanin area density, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Figure 5 from Hodges et al., 2023
eye decreased pigmentation, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Figure 2 from Hodges et al., 2023
eye decreased size, abnormal	pmela^{ua5030/ua5030} (AB)	standard conditions	Figure 3 from Hodges et al., 2023
whole organism pmela expression decreased amount, abnormal	pmela^ua5030/+ (AB)	standard conditions	Fig. S2, Figure 2 from Hodges et al., 2023
retina Melanin area density, abnormal	pmela^ua5030/+ (AB)	standard conditions	Figure 5 from Hodges et al., 2023
eye decreased size, abnormal	pmela^ua5030/+ (AB)	standard conditions	Figure 3 from Hodges et al., 2023

Citations