CRISPR

CRISPR5-arnt2

ID

ZDB-CRISPR-240620-7

Name

CRISPR5-arnt2

Previous Names

None

Target

arnt2 (1)

Sequence

5' - GGTCTCGGTCCGTCTAAGGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bcm3	arnt2

Expression

Gene expression in Wild Types + CRISPR5-arnt2

No data available

Phenotype

Phenotype resulting from CRISPR5-arnt2

No data available

Phenotype of all Fish created by or utilizing CRISPR5-arnt2

Phenotype	Fish	Conditions	Figures
heart looping decreased process quality, abnormal	arnt2^bcm3/bcm3	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Fig. 7 from Edwards et al., 2023
heart edematous, abnormal	arnt2^bcm3/bcm3	chemical treatment by environment: 2,3,7,8-tetrachlorodibenzodioxine	Fig. 7 from Edwards et al., 2023
posterior lateral mesoderm lmo2 expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
atrium increased size, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 1 from Edwards et al., 2023
dorsal aorta kdrl expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 2 from Edwards et al., 2023
anterior lateral plate mesoderm lmo2 expression decreased distribution, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
blood cell decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 1 from Edwards et al., 2023
anterior lateral plate mesoderm lmo2 expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
hematopoietic stem cell runx1 expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 2 from Edwards et al., 2023
posterior lateral mesoderm tal1 expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
posterior lateral mesoderm tal1 expression decreased distribution, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
dorsal longitudinal anastomotic vessel kdrl expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 2 from Edwards et al., 2023
anterior lateral plate mesoderm etsrp expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
posterior lateral mesoderm etsrp expression decreased distribution, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
anterior lateral plate mesoderm tal1 expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
posterior lateral mesoderm lmo2 expression decreased distribution, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
posterior lateral mesoderm etsrp expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
anterior lateral plate mesoderm etsrp expression decreased distribution, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
heart edematous, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 1 from Edwards et al., 2023
blood circulation decreased process quality, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 1 from Edwards et al., 2023
anterior lateral plate mesoderm tal1 expression decreased distribution, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3	standard conditions	Fig. 3 from Edwards et al., 2023
caudal vein plexus EGFP expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3; y1Tg/y1Tg	standard conditions	Fig. 2 from Edwards et al., 2023
intersegmental vessel EGFP expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3; y1Tg/y1Tg	standard conditions	Fig. 2 from Edwards et al., 2023
dorsal aorta EGFP expression decreased amount, abnormal	arnt^bcm2/bcm2; arnt2^bcm3/bcm3; y1Tg/y1Tg	standard conditions	Fig. 2 from Edwards et al., 2023

Citations